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Despite the difficulties discussed above, many correlations were found between gene expression levels and alcohol self-administration behaviour Table 1 ; . There were 7 correlations in the prefrontal cortex, 3 in the hippocampus and 2 in the amygdala. Looking first at the PFC, GABAA 5 was strongly correlated r 0.96, p 0.0028 ; with alcohol consumption Paper III, Figure 2 ; and the same was true for the G-protein coupled receptor GABAB1 r 0.96, p 0.0028 ; . The glutamatergic system was differentially involved in alcohol consumption. No correlation was observed for either the NMDA NR2A or NR2B subunit but AMPA GluR1 was correlated r 0.93, p 0.0067 ; . For the serotonergic system Paper III, Figure 2 ; , only the ion channel 5-HT3A was correlated r 0.93, p 0.0067 ; . All of the adrenoceptors tested 1A r 1.00, p 0.0004; 1B r 0.93, p 0.0067; 2A r 0.93, p 0.0067 ; were positively correlated to consumption Paper III, Figure 3 ; . To address whether saccharin may have confounded the above correlations, saccharin consumption from Day 5 6 was correlated to gene expression. The behaviour measured on Day 5 6 was therefore independent of alcohol since it occurred before any ethanol exposure. In the prefrontal cortex, GABAB1 was positively correlated r 0.82, p 0.034 ; to saccharin consumption Paper III, Figure 2B ; . Since this correlation was also observed for alcohol, it may not be ethanol-specific.

The exceptionally skilled technical assistance of Stacey Galdi is greatly acknowledged. We thank D. Nelson and A. Nishi for their critical reading of this manuscript. We also thank G. L. Snyder, J. A. Bibb, F. Liu, and H. C. Hemmings, Jr., for antibodies against phospho-Thr-34DARPP-32, phospho-Thr-75DARPP-32, phospho-Ser-137DARPP-32, and total DARPP32. This work was supported by National Institutes of Health Grants MH-40899 and DA10044 to P. G. ; supported by a postdoctoral fellowship from Stiftelsen for Internationalisering av Hogre Utbildning och Forskning STINT. If the individual has any concerns this must be discussed with the service manager and an appropriate action plan implemented. 9.18 Unqualified nurses who participate in the administration of medicines must adhere to this policy. SELF-ADMINISTRATION OF MEDICATION BY A PATIENT see appendix II ; Where appropriate patients should be encouraged to take full responsibility for their own medication as this can help foster feelings of independence and control. When patients take responsibility for their own medication it should not be assumed that they will be entirely responsible for their own medicines as appropriate support and supervision from nurses may be required. All service areas implementing self-administration of prescribed medication by patients must adhere to the self administration procedure attached appendix II ; MANAGEMENT OF ADVERSE INCIDENTS ADMINISTRATION ERRORS AND OR.

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