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There are other generic SSRIs available eg, fluoxetine ; . Citalopram costs the institution $0.65 to $5.11 per month based on common dosages. Tramadol [eg, Ultram] is a synthetic analog of codeine that has lower affinity for opioid receptors. It has less potential for abuse and respiratory depression. Tramadol is used for moderate pain. While tramadol does not perform well and climara. 1Department of Diagnostic Radiology. Hahnemann University College of Medicine, Broad and Vine Sts. , Philadelphia, PA 19102. Address reprint requests to E. E. Schwartz. Rhabdomyolysis refers to myopathy extensive enough to cause spillage of myoglobin into the urine; this nephrotoxic substance can induce acute renal failure and clonazepam. Subtype-specific primer pair for each of the 26 members of the Kv1Kv11 families. To obtain efficient and specific primer pairs, we used the Vector NTI program Informax, Frederick, MD ; to select sequences. Each primer sequence then was submitted to a basic local alignment search tool search against GenBank to ensure specificity of the selected sequence. Specific primer pairs were then used to amplify Kv channel subtypes from human fetal brain cDNA. Finally, the most effective primer pairs for each subtype were used to study the expression of Kv channels in human islets Table 1 ; . Antibodies. A rabbit polyclonal antibody for Kv1.6 was raised against the peptide RRSSYLPTPHRAYAEKRM, corresponding to residue 509 526 of the rat Kv1.6 34 ; . Human Kv1.6 shares 17 of 18 amino acid residues with the rat channel in this region. Rabbit polyclonal antibodies against Kv2.1 and Kv3.2 proteins were purchased from Alomone Labs Jerusalem, Israel ; . The Kv2.1 antibody was raised against the peptide HMLPGGGAHGSTRDQSI, corresponding to residue 837 853 of rat Kv2.1. Human Kv2.1 shares 15 of 17 amino acids with this region of the rat channel. The Kv3.2 antibody was raised against the peptide DLGGKRLGIEDAAGLGGPDGK C ; , corresponding to residues 184 204 of rat Kv3.2. The human and rat Kv3.2 have 19 identical amino acids in this peptide sequence. Antibodies for Kv2.1 and Kv3.2 detected only a single band in Western blots of the cognate channels expressed in HEK 293 cells. RT-PCR. Human pancreatic islets were obtained from the University of Alberta Edmonton, AB, Canada ; . The islets were purified based on staining with the -cellspecific dye diphenylthiocarbazone Sigma ; , as previously described 35 ; . The total RNA was isolated using TRI Reagent Molecular Research Center, Cincinnati, OH ; according to the manufacturer's instruction. Because most of the human Kv channel genes are intronless, we treated the total RNA with DNaseI Ambion, Austin, TX ; to eliminate traces of genomic DNA. A control PCR reaction was performed with -actin primers forward: 5 -GCCCTTTCTCACTGGTTCTC-3 ; reverse: 5 -CTTTACACCAGCCTCAT GGC-3 ; located on an intron region to verify the absence of genomic DNA. The DNaseI-digested RNA was transcribed into cDNA using SensiScript Reverse Transcriptase from Qiagen Valencia, CA ; , per the manufacturer's instruction. Human fetal brain poly A RNA was purchased from BD Biosciences Clontech Palo Alto, CA ; . The RNA was transcribed into cDNA with the Omniscript Reverse Transcriptase Kit from Qiagen following the manufacturer's instructions. The cDNA was used as templates for the amplification of individual channel subtypes. Approximately 100 ng of total RNA DIABETES, VOL. 53, MARCH 2004, for instance, cdlexa paxil.
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